Hotspot

trnM-CAU-atpE

LSC · 54469-54764 · 296 bp

Back to Gymnosporia

Pi 0.0101

MarkerSeek score 54.60

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 3
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	58.4	58.0	422 bp	1.0	77.3
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	TGGCGGAAGATAACTTGACG	50.0	50.0	58.4	58.0	455 bp	1.0	77.2
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	TTGAGGCAAATCTCGCTCTC	50.0	50.0	58.4	58.0	410 bp	1.0	77.2
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	395 bp	1.0	77.1
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	57.3	58.0	423 bp	1.0	75.3