Hotspot

ndhJ-ndhK

LSC · 51743-51869 · 127 bp

Back to Gymnosporia

Pi 0.0157

MarkerSeek score 61.60

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 2
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GAGCAGACAAACAACCTTGC	ACACAGCAGGGTCAGTATCT	50.0	50.0	57.9	58.1	190 bp	1.0	79.4
ndhJ-ndhK_p2	TCCGCGGGCTTTATTTGTAA	ACACAGCAGGGTCAGTATCT	45.0	50.0	57.9	58.1	272 bp	1.0	79.3
ndhJ-ndhK_p3	TGAACTAGCCCGTGTTTGAC	ACACAGCAGGGTCAGTATCT	50.0	50.0	58.1	58.1	215 bp	1.0	79.3
ndhJ-ndhK_p4	CCTTGGTAATCGAAGCCCAA	ACACAGCAGGGTCAGTATCT	50.0	50.0	57.9	58.1	242 bp	1.0	79.2
ndhJ-ndhK_p5	CAATCCGCGGGCTTTATTTG	ACACAGCAGGGTCAGTATCT	50.0	50.0	58.2	58.1	275 bp	1.0	78.8