Hotspot

ycf3-trnS-GGA

LSC · 45885-46815 · 931 bp

Back to Gymnosporia

Pi 0.0043

MarkerSeek score 57.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 4
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	GCTGAATTGGGTGAAAGTGC	45.0	50.0	56.4	57.9	1133 bp	1.0	67.0
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	GCTGAATTGGGTGAAAGTGC	45.0	50.0	56.4	57.9	1134 bp	1.0	67.0
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1094 bp	1.0	66.9
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1095 bp	1.0	66.9
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	GGTGAAAGTGCGGAAAGAGA	45.0	50.0	56.4	58.1	1124 bp	1.0	66.6