Hotspot

psbC-trnS-UGA

LSC · 36250-36491 · 242 bp

Back to Gymnosporia

Pi 0

MarkerSeek score 26.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 242
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCCTGTTCTCTCCATGACT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	351 bp	1.0	79.1
psbC-trnS-UGA_p2	TGAGCCTGTTCTCTCCATGA	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	353 bp	1.0	79.1
psbC-trnS-UGA_p3	AGCCTGTTCTCTCCATGACT	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	362 bp	1.0	78.6
psbC-trnS-UGA_p4	TGAGCCTGTTCTCTCCATGA	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.1	58.1	364 bp	1.0	78.5
psbC-trnS-UGA_p5	AGCCTGTTCTCTCCATGACT	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	297 bp	1.0	77.9