Hotspot

rps12-rrn16

Genome · 142771-144738 · 1968 bp

Back to Gymnosphaera

Pi 0.044

MarkerSeek score 79.30

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: Genome
length_bp: 1968
variable_sites: 170
pi: 0.044
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	CCGTTACCTCGAACTTGGAG	TCCGACTTGCATGTGTTAGG	55.0	50.0	58.0	57.9	2114-2117 bp	0.75	69.6
rps12-rrn16_p2	CCGTTACCTCGAACTTGGAG	AACACCATGAGGATGCTTCC	55.0	50.0	58.0	57.9	2136-2139 bp	0.75	69.2
rps12-rrn16_p3	CCGTTACCTCGAACTTGGAG	GGAAACACCATGAGGATGCT	55.0	50.0	58.0	57.9	2139-2142 bp	0.75	69.2
rps12-rrn16_p4	GAACTCTGGCGACTTTACGT	TCCGACTTGCATGTGTTAGG	50.0	50.0	57.9	57.9	2087-2090 bp	0.75	69.1
rps12-rrn16_p5	ACGTAGGGCAGAGTTAGGTT	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	2071-2074 bp	0.75	69.1