Hotspot

psbA-rps7

Genome · 140550-141420 · 871 bp

Back to Gymnosphaera

Pi 0.00344
MarkerSeek score 61.20
Primer available yes
Label psbA-rps7

Feature Metadata

label_name
psbA-rps7
feature_type
igs
region
Genome
length_bp
871
variable_sites
6
pi
0.00344
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-rps7_p1 CGCGTCTTTCTAAAGTTGCG TGAATCAATGGATGCTGCCA 50.0 45.0 58.1 57.9 999-1016 bp 1.0 78.1
psbA-rps7_p2 TAGACGGTTTTCAGTGCTGG TGAATCAATGGATGCTGCCA 50.0 45.0 57.8 57.9 1058-1075 bp 1.0 78.0
psbA-rps7_p3 CGCGTCTTTCTAAAGTTGCG TCAATGGATGCTGCCAGAAA 50.0 45.0 58.1 57.8 995-1012 bp 1.0 77.6
psbA-rps7_p4 GCGTCTTTCTAAAGTTGCGG TGAATCAATGGATGCTGCCA 50.0 45.0 57.8 57.9 998-1015 bp 1.0 77.5
psbA-rps7_p5 TAGACGGTTTTCAGTGCTGG TCAATGGATGCTGCCAGAAA 50.0 45.0 57.8 57.8 1054-1071 bp 1.0 77.4