Hotspot

rps15-ycf1

Genome · 123230-123576 · 347 bp

Back to Gymnosphaera

Pi 0.0212

MarkerSeek score 61.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: Genome
length_bp: 347
variable_sites: 13
pi: 0.0212
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GTTCTCATCCATTAACGACA	TCGATTTGCCATGCTCAAGA	40.0	45.0	53.1	57.9	392-430 bp	1.0	46.5
rps15-ycf1_p2	TCTCATCCATTAACGACAAATCA	TCGATTTGCCATGCTCAAGA	34.8	45.0	56.0	57.9	390-428 bp	1.0	46.5
rps15-ycf1_p3	GTTCTCATCCATTAACGACA	AAGTCGATTTGCCATGCTCA	40.0	45.0	53.1	58.2	395-433 bp	1.0	46.5
rps15-ycf1_p4	GTTCTCATCCATTAACGACA	AGTCGATTTGCCATGCTCAA	40.0	45.0	53.1	58.2	394-432 bp	1.0	46.5
rps15-ycf1_p5	GTTCTCATCCATTAACGACA	GCCATGCTCAAGATACGGAT	40.0	50.0	53.1	57.8	385-423 bp	1.0	46.5