Hotspot

psaC-ndhE

Genome · 117410-117785 · 376 bp

Back to Gymnosphaera

Pi 0.0372

MarkerSeek score 75.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: Genome
length_bp: 376
variable_sites: 26
pi: 0.0372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTACGCACTGAGTACAACCG	GCCCTCGCTCTTGTTATTCA	50.0	50.0	57.9	58.0	502-505 bp	1.0	83.8
psaC-ndhE_p2	TTACGCACTGAGTACAACCG	CTGCTGAAGCTGCTATTGGA	50.0	50.0	57.9	58.0	525-528 bp	1.0	83.5
psaC-ndhE_p3	CCAACACATCTGTGGGACAT	GCCCTCGCTCTTGTTATTCA	50.0	50.0	57.8	58.0	526-529 bp	1.0	82.4
psaC-ndhE_p4	CCAACACATCTGTGGGACAT	CTGCTGAAGCTGCTATTGGA	50.0	50.0	57.8	58.0	549-552 bp	1.0	82.2
psaC-ndhE_p5	TTACGCACTGAGTACAACCG	CTGCTATTGGATTGGCCCTC	50.0	55.0	57.9	58.4	516-519 bp	1.0	80.8