Hotspot

trnP-GGG-trnL-UAG

Genome · 113642-113823 · 182 bp

Back to Gymnosphaera

Pi 0.0266

MarkerSeek score 55.40

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 182
variable_sites: 9
pi: 0.0266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GCTACCAAGCTGCGCTATAT	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	232-241 bp	1.0	83.7
trnP-GGG-trnL-UAG_p2	GCTACCAAGCTGCGCTATAT	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	233-242 bp	1.0	83.6
trnP-GGG-trnL-UAG_p3	ACCAAGCTGCGCTATATTCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	229-238 bp	1.0	83.5
trnP-GGG-trnL-UAG_p4	ACCAAGCTGCGCTATATTCC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	230-239 bp	1.0	83.4
trnP-GGG-trnL-UAG_p5	GCTACCAAGCTGCGCTATAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	242-251 bp	1.0	82.5