Hotspot

rpl32-trnP-GGG

Genome · 112946-113567 · 622 bp

Back to Gymnosphaera

Pi 0.0255

MarkerSeek score 61.60

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 622
variable_sites: 29
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	AGCTTTTTCTTCAGCCCAGT	TCGTATGGGGGTAACGGAAT	45.0	50.0	57.6	58.2	781-801 bp	1.0	80.0
rpl32-trnP-GGG_p2	AGCTTTTTCTTCAGCCCAGT	GGGGTAACGGAATATAGCGC	45.0	55.0	57.6	58.3	774-794 bp	1.0	79.4
rpl32-trnP-GGG_p3	AGCTTTTTCTTCAGCCCAGT	TACTCGTATGGGGGTAACGG	45.0	55.0	57.6	58.3	784-804 bp	1.0	79.2
rpl32-trnP-GGG_p4	AGCTTTTTCTTCAGCCCAGT	GGGGGTAACGGAATATAGCG	45.0	55.0	57.6	57.6	775-795 bp	1.0	78.7
rpl32-trnP-GGG_p5	AGCTTTTTCTTCAGCCCAGT	GGGTAACGGAATATAGCGCA	45.0	50.0	57.6	57.6	773-793 bp	1.0	78.2