Hotspot

rrn16-rps12

Genome · 96338-98305 · 1968 bp

Back to Gymnosphaera

Pi 0.044

MarkerSeek score 79.30

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: Genome
length_bp: 1968
variable_sites: 170
pi: 0.044
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	TCCGACTTGCATGTGTTAGG	CCGTTACCTCGAACTTGGAG	50.0	55.0	57.9	58.0	2114-2117 bp	0.75	69.6
rrn16-rps12_p2	AACACCATGAGGATGCTTCC	CCGTTACCTCGAACTTGGAG	50.0	55.0	57.9	58.0	2136-2139 bp	0.75	69.2
rrn16-rps12_p3	GGAAACACCATGAGGATGCT	CCGTTACCTCGAACTTGGAG	50.0	55.0	57.9	58.0	2139-2142 bp	0.75	69.2
rrn16-rps12_p4	TCCGACTTGCATGTGTTAGG	GAACTCTGGCGACTTTACGT	50.0	50.0	57.9	57.9	2087-2090 bp	0.75	69.1
rrn16-rps12_p5	TCCGACTTGCATGTGTTAGG	ACGTAGGGCAGAGTTAGGTT	50.0	50.0	57.9	58.1	2071-2074 bp	0.75	69.1