Hotspot

rpl2-rpl23

Genome · 85141-85202 · 62 bp

Back to Gymnosphaera

Pi 0.0376

MarkerSeek score 68.10

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: Genome
length_bp: 62
variable_sites: 4
pi: 0.0376
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CTGACCGTCACATCGAATGT	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.0	58.0	1301-1306 bp	1.0	82.3
rpl2-rpl23_p2	CTGACCGTCACATCGAATGT	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.0	58.0	1302-1307 bp	1.0	82.3
rpl2-rpl23_p3	CTGACCGTCACATCGAATGT	ACGCCGCAAAAGAATGATTG	50.0	45.0	58.0	58.0	829-832 bp	1.0	81.7
rpl2-rpl23_p4	CTGACCGTCACATCGAATGT	CGCCGCAAAAGAATGATTGT	50.0	45.0	58.0	58.0	828-831 bp	1.0	81.7
rpl2-rpl23_p5	CTGACCGTCACATCGAATGT	CGGGTCGAAGAAGAAGACAG	50.0	55.0	58.0	58.0	884 bp	0.5	59.9