Hotspot

petG-trnW-CCA

Genome · 65270-65589 · 320 bp

Back to Gymnosphaera

Pi 0.0401

MarkerSeek score 72.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 320
variable_sites: 24
pi: 0.0401
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGCGGTGACCAATTAGAAA	AAGCGCTCTTAGTTCAGTCC	45.0	50.0	57.8	57.6	419-424 bp	1.0	81.1
petG-trnW-CCA_p2	ATTCACAATATCGACGCGGT	AAGCGCTCTTAGTTCAGTCC	45.0	50.0	57.8	57.6	432-437 bp	1.0	80.6
petG-trnW-CCA_p3	GACGCGGTGACCAATTAGAA	AAGCGCTCTTAGTTCAGTCC	50.0	50.0	58.3	57.6	420-425 bp	1.0	80.2
petG-trnW-CCA_p4	ACGCGGTGACCAATTAGAAA	GTTCGAATCCTACAGGGCG	45.0	57.9	57.8	58.0	364-369 bp	1.0	76.7
petG-trnW-CCA_p5	TTCACAATATCGACGCGGTG	AAGCGCTCTTAGTTCAGTCC	50.0	50.0	58.7	57.6	431-436 bp	1.0	76.6