Hotspot

petA-psbJ

Genome · 62314-63045 · 732 bp

Back to Gymnosphaera

Pi 0.0143

MarkerSeek score 65.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 732
variable_sites: 20
pi: 0.0143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAGATCTTTCTCGTG	TACACTTGTGATAGGCTCGC	50.0	50.0	57.9	57.4	863-898 bp	1.0	77.5
petA-psbJ_p2	TCCAGTTGGCGGAAATGAAT	TACACTTGTGATAGGCTCGC	45.0	50.0	57.8	57.4	818-853 bp	1.0	76.6
petA-psbJ_p3	TGGCACAGATCTTTCTCGTG	CACTTGTGATAGGCTCGCTG	50.0	55.0	57.9	58.7	861-896 bp	1.0	76.4
petA-psbJ_p4	GCACAGATCTTTCTCGTGCT	TACACTTGTGATAGGCTCGC	50.0	50.0	58.3	57.4	861-896 bp	1.0	76.0
petA-psbJ_p5	CCGTTGCGTATTCAAGGTCT	TACACTTGTGATAGGCTCGC	50.0	50.0	58.3	57.4	909-944 bp	1.0	75.8