Hotspot

cemA-petA

Genome · 61119-61347 · 229 bp

Back to Gymnosphaera

Pi 0.0378

MarkerSeek score 66.40

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: Genome
length_bp: 229
variable_sites: 15
pi: 0.0378
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGCTTCGTTTCAACCTTTCC	ATGGGCAGTTTGCTTTACCA	50.0	45.0	58.0	58.0	437-452 bp	1.0	82.4
cemA-petA_p2	GCTTCGTTTCAACCTTTCCG	ATGGGCAGTTTGCTTTACCA	50.0	45.0	58.0	58.0	436-451 bp	1.0	82.4
cemA-petA_p3	TCTCGCTTCGTTTCAACCTT	ATGGGCAGTTTGCTTTACCA	45.0	45.0	57.8	58.0	440-455 bp	1.0	80.8
cemA-petA_p4	CGCTTCGTTTCAACCTTTCC	TGGGCAGTTTGCTTTACCAA	50.0	45.0	58.0	58.2	436-451 bp	1.0	80.7
cemA-petA_p5	GCTTCGTTTCAACCTTTCCG	TGGGCAGTTTGCTTTACCAA	50.0	45.0	58.0	58.2	435-450 bp	1.0	80.7