Hotspot

psaM-ycf12

Genome · 8611-8854 · 244 bp

Back to Gymnosphaera

Pi 0.0287

MarkerSeek score 61.30

Primer available yes

Label psaM-ycf12

Feature Metadata

label_name: psaM-ycf12
feature_type: igs
region: Genome
length_bp: 244
variable_sites: 12
pi: 0.0287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-ycf12_p1	GCAATAGCCAATCTCGCAAC	ACTATTAGCGCCAGAACAGC	50.0	50.0	57.9	58.1	365-413 bp	1.0	83.7
psaM-ycf12_p2	GCAATAGCCAATCTCGCAAC	CTATTAGCGCCAGAACAGCA	50.0	50.0	57.9	58.1	364-412 bp	1.0	83.7
psaM-ycf12_p3	GCAATAGCCAATCTCGCAAC	GTGGTCCCGATGCAACTATT	50.0	50.0	57.9	58.0	379-427 bp	1.0	83.6
psaM-ycf12_p4	GCAATAGCCAATCTCGCAAC	TAGTGGTCCCGATGCAACTA	50.0	50.0	57.9	58.1	381-429 bp	1.0	82.8
psaM-ycf12_p5	GCAATAGCCAATCTCGCAAC	TACTAGTGGTCCCGATGCAA	50.0	50.0	57.9	58.1	384-432 bp	1.0	82.7