Hotspot

matK-rps16

Genome · 3020-4156 · 1137 bp

Back to Gymnosphaera

Pi 0.0166

MarkerSeek score 45.80

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 1137
variable_sites: 34
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GAATCTGCAGTCCAGCTACC	GGGCAAAGGTACCTGAACAA	55.0	50.0	58.1	58.0	1459-1467 bp	1.0	81.7
matK-rps16_p2	GAATCTGCAGTCCAGCTACC	TTCTGTTGCTGCCAATTCCT	55.0	45.0	58.1	58.0	2018-2036 bp	1.0	81.5
matK-rps16_p3	GAATCTGCAGTCCAGCTACC	GCGAGAGGGAAAAGCCATTA	55.0	50.0	58.1	58.0	1596-1604 bp	1.0	81.4
matK-rps16_p4	GAATCTGCAGTCCAGCTACC	TTCATGATCCGCGGAAAGAG	55.0	50.0	58.1	58.1	1564-1572 bp	1.0	81.3
matK-rps16_p5	GAATCTGCAGTCCAGCTACC	TGGGGTTAGGCTCAAACCTA	55.0	50.0	58.1	58.0	1983-2001 bp	1.0	81.2