Hotspot

ndhG-ndhI

SSC · 121658-122167 · 510 bp

Back to Gymnospermium

Pi 0.0303

MarkerSeek score 67.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 510
variable_sites: 41
pi: 0.0303
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CGCCGACTATAAGACCCAAC	GGTAAACCCCTTGATTCAATAACA	55.0	37.5	58.2	57.2	598-783 bp	1.0	49.0
ndhG-ndhI_p2	CGCCGACTATAAGACCCAAC	GGTAAACCCCTTGATTCAAT	55.0	40.0	58.2	53.1	598-783 bp	1.0	49.0
ndhG-ndhI_p3	GCCGACTATAAGACCCAACC	GGTAAACCCCTTGATTCAATAACA	55.0	37.5	57.8	57.2	597-782 bp	1.0	49.0
ndhG-ndhI_p4	GCCGACTATAAGACCCAACC	GGTAAACCCCTTGATTCAAT	55.0	40.0	57.8	53.1	597-782 bp	1.0	49.0
ndhG-ndhI_p5	AATACTACTCCTAGCCCGCC	GGTAAACCCCTTGATTCAATAACA	55.0	37.5	58.4	57.2	614-799 bp	1.0	48.9