Hotspot

ccsA-ndhD

SSC · 118090-118390 · 301 bp

Back to Gymnospermium

Pi 0.052

MarkerSeek score 74.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 301
variable_sites: 47
pi: 0.052
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAATATGGTGCCCATGGTGC	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1193-1238 bp	0.375	56.7
ccsA-ndhD_p2	TAATATGGTGCCCATGGTGC	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1194-1239 bp	0.375	56.7
ccsA-ndhD_p3	TAATATGGTGCCCATGGTGC	CAATGGCTTCCCTTGCATTG	50.0	50.0	58.0	58.0	1417-1462 bp	0.375	56.4
ccsA-ndhD_p4	TAATATGGTGCCCATGGTGC	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.0	58.1	1418-1463 bp	0.375	56.0
ccsA-ndhD_p5	TAATATGGTGCCCATGGTGC	CTCTCGGTCAACGCAACTTA	50.0	50.0	58.0	57.9	1678-1723 bp	0.375	55.8