Hotspot

ndhF-rpl32

SSC · 114732-115921 · 1190 bp

Back to Gymnospermium

Pi 0.0224

MarkerSeek score 69.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1190
variable_sites: 76
pi: 0.0224
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAGGGT	AGTACGTTTCTTCGGAACCG	50.0	50.0	57.1	57.9	955-1266 bp	1.0	81.6
ndhF-rpl32_p2	TGAAGGGTATGATCCATGCA	AGTACGTTTCTTCGGAACCG	45.0	50.0	56.3	57.9	943-1254 bp	1.0	74.9
ndhF-rpl32_p3	CTGGAAGTGGAATGAAGGGTA	AGTACGTTTCTTCGGAACCG	47.6	50.0	57.0	57.9	955-1266 bp	1.0	72.7
ndhF-rpl32_p4	CTGGAAGTGGAATGAAGGGT	TACGTTTCTTCGGAACCGC	50.0	52.6	57.1	58.2	953-1264 bp	1.0	72.5
ndhF-rpl32_p5	TGAAGGGTATGATCCATGCAT	AGTACGTTTCTTCGGAACCG	42.9	50.0	56.8	57.9	943-1254 bp	1.0	71.3