Hotspot

ycf1-ndhF

SSC · 112422-112580 · 159 bp

Back to Gymnospermium

Pi 0.253

MarkerSeek score 69.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 159
variable_sites: 88
pi: 0.253
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	TTGCCGCTTCTTGTATTGGT	50.0	45.0	58.0	58.1	1017-1657 bp	1.0	91.1
ycf1-ndhF_p2	ACGAAGGGGACTAAACAGGA	TGGGGCAAAAATGGAGTGAA	50.0	45.0	58.0	57.9	1146-1786 bp	1.0	91.0
ycf1-ndhF_p3	CCTTCCCCTTCCCTTTGTTC	TGGGGCAAAAATGGAGTGAA	55.0	45.0	58.1	57.9	1101-1741 bp	1.0	90.6
ycf1-ndhF_p4	TTTAGGCAGAATACCGTCGC	TTGCCGCTTCTTGTATTGGT	50.0	45.0	58.1	58.1	1132-1772 bp	1.0	90.3
ycf1-ndhF_p5	TTTAGGCAGAATACCGTCGC	TGGGGCAAAAATGGAGTGAA	50.0	45.0	58.1	57.9	1261-1901 bp	1.0	89.9