Hotspot

rpl14-rpl16

LSC · 82884-83007 · 124 bp

Back to Gymnospermium

Pi 0.0564
MarkerSeek score 69.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
124
variable_sites
14
pi
0.0564
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AATAGGTTTGAGGTTGAATCA AAATTGCAGCCAGAGAAGCT 33.3 45.0 52.7 58.1 222-226 bp 1.0 47.2
rpl14-rpl16_p2 ATAGGTTTGAGGTTGAATCAT AAATTGCAGCCAGAGAAGCT 33.3 45.0 52.5 58.1 221-225 bp 1.0 47.2
rpl14-rpl16_p3 AAATAGGTTTGAGGTTGAATCA AAATTGCAGCCAGAGAAGCT 31.8 45.0 53.6 58.1 223-227 bp 1.0 47.1
rpl14-rpl16_p4 AATAGGTTTGAGGTTGAATCA GAAATTGCAGCCAGAGAAGC 33.3 50.0 52.7 57.7 223-227 bp 1.0 47.1
rpl14-rpl16_p5 AAATAGGTTTGAGGTTGAATCA GAAATTGCAGCCAGAGAAGC 31.8 50.0 53.6 57.7 224-228 bp 1.0 47.1