Hotspot

psbZ-trnG-UCC

LSC · 37489-38000 · 512 bp

Back to Gymnospermium

Pi 0.0438

MarkerSeek score 74.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 53
pi: 0.0438
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CGGATAGATGCACGATCCAG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	1175-1462 bp	1.0	87.6
psbZ-trnG-UCC_p2	CGGATAGATGCACGATCCAG	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.0	58.0	1497-1784 bp	1.0	87.1
psbZ-trnG-UCC_p3	GCCAGGCGAAACAAGAAATC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1467-1741 bp	1.0	87.1
psbZ-trnG-UCC_p4	TGGCTCAGAGAGGGATCAAA	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1350-1602 bp	1.0	87.1
psbZ-trnG-UCC_p5	GCCAGGCGAAACAAGAAATC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1789-2063 bp	1.0	86.9