Hotspot

trnT-GGU-psbD

LSC · 32563-34232 · 1670 bp

Back to Gymnospermium

Pi 0.0147

MarkerSeek score 61.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1670
variable_sites: 69
pi: 0.0147
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1808-1935 bp	1.0	84.1
trnT-GGU-psbD_p2	ATGGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1812-1939 bp	1.0	83.8
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	50.0	50.0	57.9	57.6	1807-1934 bp	1.0	83.0
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1771-1898 bp	1.0	82.8
trnT-GGU-psbD_p5	ATGGGCCCTTTTAACTCAGC	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1811-1938 bp	1.0	82.6