Hotspot

psbM-trnD-GUC

LSC · 29999-31230 · 1232 bp

Back to Gymnospermium

Pi 0.0088

MarkerSeek score 61.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 34
pi: 0.0088
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAATGCAGTAGC	AGTTCAATCGGTCAGAGCAC	40.0	50.0	54.0	57.9	1341-1348 bp	1.0	50.2
psbM-trnD-GUC_p2	AGAATGAACAATGCAGTAGC	GTTCAATCGGTCAGAGCACC	40.0	55.0	54.0	58.9	1340-1347 bp	1.0	43.5
psbM-trnD-GUC_p3	TAGAATGAACAATGCAGTAGC	AGTTCAATCGGTCAGAGCAC	38.1	50.0	54.0	57.9	1342-1349 bp	1.0	43.0
psbM-trnD-GUC_p4	AGGAATTAGAATGAACAATGCAGT	AGTTCAATCGGTCAGAGCAC	33.3	50.0	56.9	57.9	1348-1355 bp	1.0	43.0
psbM-trnD-GUC_p5	AGAATGAACAATGCAGTAGC	TAGTTCAATCGGTCAGAGCAC	40.0	47.6	54.0	57.8	1342-1349 bp	1.0	43.0