Hotspot

atpH-atpI

LSC · 13866-15020 · 1155 bp

Back to Gymnospermium

Pi 0.00786

MarkerSeek score 61.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1155
variable_sites: 28
pi: 0.00786
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1072-1354 bp	1.0	85.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1029-1311 bp	1.0	85.0
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1085-1367 bp	1.0	84.3
atpH-atpI_p4	CAATAGAAGCCAGCCCTACG	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.1	57.9	1064-1346 bp	1.0	84.2
atpH-atpI_p5	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.1	57.9	1114-1396 bp	1.0	84.1