Hotspot

trnK-UUU-rps16

LSC · 4799-5606 · 808 bp

Back to Gymnospermium

Pi 0.0201

MarkerSeek score 60.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 48
pi: 0.0201
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCCTTTCAGGATCAGTCGTG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1003-1222 bp	1.0	85.9
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1002-1221 bp	1.0	85.9
trnK-UUU-rps16_p3	CCCTTTCAGGATCAGTCGTG	AAAGGGGGTTAGAGACAGCT	55.0	50.0	58.0	58.0	1438-1687 bp	1.0	85.6
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AAAGGGGGTTAGAGACAGCT	55.0	50.0	58.0	58.0	1437-1686 bp	1.0	85.6
trnK-UUU-rps16_p5	CCTTGCGGCTCTATCCATTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1158-1377 bp	1.0	85.3