Hotspot

trnH-GUG-psbA

LSC · 77-939 · 863 bp

Back to Gymnospermium

Pi 0.0356

MarkerSeek score 68.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 80
pi: 0.0356
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	AAGCTCCATCTACGAATGGC	50.0	50.0	57.9	57.8	790-914 bp	1.0	88.4
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	AAGCTCCATCTACGAATGGC	50.0	50.0	58.2	57.8	791-915 bp	1.0	88.2
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	AAGCTCCATCTACGAATGGC	50.0	50.0	57.7	57.8	783-907 bp	1.0	86.9
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	AGCTCCATCTACGAATGGCT	50.0	50.0	57.9	58.6	789-913 bp	1.0	85.8
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	AAGCTCCATCTACGAATGGC	50.0	50.0	57.5	57.8	812-936 bp	1.0	85.5