Hotspot

ndhD

SSC · 130907-132400 · 1494 bp

Back to Gymnocladus

Pi 0.488

MarkerSeek score 79.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1494
variable_sites: 575
pi: 0.488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAACAACTCGAAGCATGGGT	AATGAGGCCTGGGGATCATA	45.0	50.0	58.0	57.9	2157 bp	0.5	58.7
ndhD_p2	AAACAACTCGAAGCATGGGT	TAATGAGGCCTGGGGATCAT	45.0	50.0	58.0	57.9	2158 bp	0.5	58.7
ndhD_p3	AAACAACTCGAAGCATGGGT	TGAGGCCTGGGGATCATATT	45.0	50.0	58.0	57.9	2155 bp	0.5	58.7
ndhD_p4	GGCATGAAACAACTCGAAGC	AATGAGGCCTGGGGATCATA	50.0	50.0	58.0	57.9	2163 bp	0.5	58.6
ndhD_p5	GGCATGAAACAACTCGAAGC	TGAGGCCTGGGGATCATATT	50.0	50.0	58.0	57.9	2161 bp	0.5	58.6