Hotspot

ndhF-trnN-GUU

IRa · 138307-140222 · 1916 bp

Back to Gymnocladus

Pi 0.174

MarkerSeek score 82.50

Primer available yes

Label ndhF-trnN-GUU

Feature Metadata

label_name: ndhF-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1916
variable_sites: 318
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN-GUU_p1	AATATGTAGGGGGTGGCAGA	CGGGAGAATTGATGACTGCA	50.0	50.0	57.8	58.0	2354 bp	0.5	58.1
ndhF-trnN-GUU_p2	ATATGTAGGGGGTGGCAGAA	CGGGAGAATTGATGACTGCA	50.0	50.0	57.8	58.0	2353 bp	0.5	58.1
ndhF-trnN-GUU_p3	TATGTAGGGGGTGGCAGAAT	CGGGAGAATTGATGACTGCA	50.0	50.0	57.8	58.0	2352 bp	0.5	58.1
ndhF-trnN-GUU_p4	ATATGTAGGGGGTGGCAGAA	GGCAGAATAGCAGAGCAAGT	50.0	50.0	57.8	58.0	2444 bp	0.5	58.1
ndhF-trnN-GUU_p5	TATGTAGGGGGTGGCAGAAT	GGCAGAATAGCAGAGCAAGT	50.0	50.0	57.8	58.0	2443 bp	0.5	58.1