Hotspot

trnL-UAG-rpl32

SSC · 133830-134885 · 1056 bp

Back to Gymnocladus

Pi 0.422

MarkerSeek score 81.20

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1056
variable_sites: 386
pi: 0.422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGGATATTGGGCAGCATTGA	50.0	50.0	58.0	58.0	1185 bp	0.5	59.9
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGGATATTGGGCAGCATTGA	50.0	50.0	58.0	58.0	1186 bp	0.5	59.9
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AGGGATATTGGGCAGCATTG	50.0	50.0	58.0	58.0	1186 bp	0.5	59.9
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AGGGATATTGGGCAGCATTG	50.0	50.0	58.0	58.0	1187 bp	0.5	59.9
trnL-UAG-rpl32_p5	TTGATACGACCGAAAACGCT	GGGATATTGGGCAGCATTGA	45.0	50.0	57.9	58.0	1395 bp	0.5	59.4