Hotspot

ndhI-ndhG

SSC · 128265-129175 · 911 bp

Back to Gymnocladus

Pi 0.354

MarkerSeek score 79.30

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 911
variable_sites: 257
pi: 0.354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AACAAACAGTACGAGCTGCA	GTGCTGCAGCTACAAAATGG	45.0	50.0	58.1	58.0	1544 bp	0.5	59.5
ndhI-ndhG_p2	ACAAACAGTACGAGCTGCAA	GTGCTGCAGCTACAAAATGG	45.0	50.0	58.1	58.0	1543 bp	0.5	59.5
ndhI-ndhG_p3	AACAAACAGTACGAGCTGCA	CAAGAACCAGTCCCAACGAA	45.0	50.0	58.1	58.0	1490 bp	0.5	59.3
ndhI-ndhG_p4	ACAAACAGTACGAGCTGCAA	CAAGAACCAGTCCCAACGAA	45.0	50.0	58.1	58.0	1489 bp	0.5	59.3
ndhI-ndhG_p5	AATCAAATTGCTTTGGGCCG	GTGCTGCAGCTACAAAATGG	45.0	50.0	57.9	58.0	1170 bp	0.5	59.1