Hotspot

rpl33-rps18

LSC · 74014-74260 · 247 bp

Back to Gymnocladus

Pi 0.127

MarkerSeek score 86.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 28
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	362-383 bp	1.0	86.9
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	363-384 bp	1.0	86.9
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	364-385 bp	1.0	85.0
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	359-380 bp	1.0	83.9
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	360-381 bp	1.0	83.9