Hotspot

trnQ-UUG-psbK

LSC · 8010-8676 · 667 bp

Back to Gymnocladus

Pi 0.0713

MarkerSeek score 84.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 667
variable_sites: 40
pi: 0.0713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCGCG	55.0	50.0	57.6	58.6	736-835 bp	1.0	79.5
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	GGCTTCAGGTAATTTCGCGA	55.0	50.0	57.6	58.6	735-834 bp	1.0	79.4
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.7	58.6	694-793 bp	1.0	77.8
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.7	58.6	693-792 bp	1.0	77.8
trnQ-UUG-psbK_p5	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.2	58.6	740-839 bp	1.0	76.2