Hotspot

ycf1

SSC · 130414-136287 · 5874 bp

Back to Gymnema

Pi 0.00678

MarkerSeek score 54.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5874
variable_sites: 57
pi: 0.00678
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTCGTTTGGCGGATTCGTA	AGAAGAAGCGGAAAAGGTGG	45.0	50.0	58.0	58.1	209-359 bp	1.0	85.4
ycf1_p2	ATTCGTTTGGCGGATTCGTA	TCCAACAACAGAAAGGCGAA	45.0	45.0	58.0	58.0	907-1042 bp	1.0	84.9
ycf1_p3	ATTCGTTTGGCGGATTCGTA	TTCCAACAACAGAAAGGCGA	45.0	45.0	58.0	58.0	908-1043 bp	1.0	84.9
ycf1_p4	AGCGCGCATAGAACCTTTAA	TCCAACAACAGAAAGGCGAA	45.0	45.0	58.0	58.0	975-1110 bp	1.0	84.8
ycf1_p5	AGCGCGCATAGAACCTTTAA	TTCCAACAACAGAAAGGCGA	45.0	45.0	58.0	58.0	976-1111 bp	1.0	84.8