Hotspot

petB

LSC · 81541-82935 · 1395 bp

Back to Gymnema

Pi 0.0119

MarkerSeek score 55.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 24
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGTTCGATCGCGGAATTTCT	TCCTTCCCCAAGTGATAAATGA	45.0	40.9	57.7	56.8	1488 bp	1.0	53.2
petB_p2	AGTTCGATCGCGGAATTTCT	TTCCTTCCCCAAGTGATAAATGA	45.0	39.1	57.7	57.5	1489 bp	1.0	50.7
petB_p3	GAGTTCGATCGCGGAATTTC	TCCTTCCCCAAGTGATAAATGA	50.0	40.9	57.4	56.8	1489 bp	1.0	50.5
petB_p4	GGAGTTCGATCGCGGAATTT	TCCTTCCCCAAGTGATAAATGA	50.0	40.9	58.7	56.8	1490 bp	1.0	49.8
petB_p5	CGATCGCGGAATTTCTTTGT	TCCTTCCCCAAGTGATAAATGA	45.0	40.9	57.2	56.8	1484 bp	1.0	49.1