Hotspot

accD

LSC · 63784-65673 · 1890 bp

Back to Gymnema

Pi 0.00955

MarkerSeek score 62.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1890
variable_sites: 27
pi: 0.00955
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATGCTAGCGATTGGGTTTCT	AGGTCACCAAACAAAATTCCT	45.0	38.1	57.3	55.9	2029-2053 bp	1.0	51.2
accD_p2	AATGCTAGCGATTGGGTTTCT	AGGTCACCAAACAAAATTCCT	42.9	38.1	58.0	55.9	2030-2054 bp	1.0	48.9
accD_p3	ATGCTAGCGATTGGGTTTCT	AGGTCACCAAACAAAATTCC	45.0	40.0	57.3	54.3	2029-2053 bp	1.0	46.3
accD_p4	ATGCTAGCGATTGGGTTTCT	GGTCACCAAACAAAATTCCT	45.0	40.0	57.3	54.3	2028-2052 bp	1.0	46.3
accD_p5	TGCTAGCGATTGGGTTTCTA	AGGTCACCAAACAAAATTCCT	45.0	38.1	56.6	55.9	2028-2052 bp	1.0	46.0