Hotspot

trnL-CAA-ycf2

IRa · 151120-151682 · 563 bp

Back to Gymnema

Pi 0.00474

MarkerSeek score 61.20

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 563
variable_sites: 4
pi: 0.00474
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	671-672 bp	1.0	78.7
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	670-671 bp	1.0	78.7
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	673-674 bp	1.0	78.7
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	672-673 bp	1.0	78.7
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	50.0	50.0	58.0	58.2	674-675 bp	1.0	78.7