Hotspot

ndhI-ndhA

SSC · 126181-126267 · 87 bp

Back to Gymnema

Pi 0.023

MarkerSeek score 67.20

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 87
variable_sites: 3
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	AGACAGGGTAATCGCGAAAC	ACAACTTCGTCCCAACTTCT	50.0	45.0	58.0	57.0	210 bp	1.0	73.1
ndhI-ndhA_p2	TGGCAGCTCGTATTGTTTGT	ACAACTTCGTCCCAACTTCT	45.0	45.0	58.1	57.0	175 bp	1.0	72.5
ndhI-ndhA_p3	CCTTGACCGATGTATCTGGC	ACAACTTCGTCCCAACTTCT	55.0	45.0	58.1	57.0	191 bp	1.0	72.2
ndhI-ndhA_p4	AGACAGGGTAATCGCGAAAC	TCGTCCCAACTTCTTTCACTG	50.0	47.6	58.0	58.2	204 bp	1.0	71.8
ndhI-ndhA_p5	AGGGTAATCGCGAAACCTTG	ACAACTTCGTCCCAACTTCT	50.0	45.0	58.3	57.0	206 bp	1.0	71.0