Hotspot

rpl32-trnL-UAG

SSC · 119866-120720 · 855 bp

Back to Gymnema

Pi 0.0225
MarkerSeek score 70.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
855
variable_sites
28
pi
0.0225
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGAAGGGATATTGGAAAGCA TTAGAAGATGACATGCCGCC 45.0 50.0 55.1 58.0 1034-1044 bp 1.0 61.9
rpl32-trnL-UAG_p2 GGAAGGGATATTGGAAAGCA TCGAACCGAGATGCTTTAGC 45.0 50.0 55.1 58.1 1006-1016 bp 1.0 61.8
rpl32-trnL-UAG_p3 GGAAGGGATATTGGAAAGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.1 57.9 980-990 bp 1.0 61.5
rpl32-trnL-UAG_p4 GGAAGGGATATTGGAAAGCA AGCAGCGTGTCTACCAATTT 45.0 45.0 55.1 57.8 973-983 bp 1.0 61.1
rpl32-trnL-UAG_p5 GGAAGGGATATTGGAAAGCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.1 57.8 978-988 bp 1.0 61.0