Hotspot

psbE-petL

LSC · 71129-72241 · 1113 bp

Back to Gymnema

Pi 0.0048

MarkerSeek score 62.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 8
pi: 0.0048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCA	45.0	45.0	58.0	56.9	1268-1271 bp	1.0	71.4
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCCGCTAGTAGAAAACCAA	45.0	45.0	58.0	56.9	1267-1270 bp	1.0	71.4
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1257-1260 bp	1.0	71.1
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1256-1259 bp	1.0	71.1
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1179-1182 bp	1.0	70.3