Hotspot

rbcL-accD

LSC · 63059-63783 · 725 bp

Back to Gymnema

Pi 0.00856

MarkerSeek score 68.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 8
pi: 0.00856
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CTGCGCTCTAACTCCTTCTT	55.0	50.0	58.0	57.3	901-925 bp	1.0	78.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	CTGCGCTCTAACTCCTTCTT	50.0	50.0	57.9	57.3	871-895 bp	1.0	78.2
rbcL-accD_p3	AAATGGAGTCCTGAACTGGC	CTGCGCTCTAACTCCTTCTT	50.0	50.0	57.8	57.3	892-916 bp	1.0	77.4
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TTTATTTAGCCCGCACCTGC	55.0	50.0	58.0	58.9	917-941 bp	1.0	76.7
rbcL-accD_p5	GGCTAGCAAATGGAGTCCTG	CTGCGCTCTAACTCCTTCTT	55.0	50.0	58.3	57.3	899-923 bp	1.0	76.4