Hotspot

ndhC-trnV-UAC

LSC · 56411-57885 · 1475 bp

Back to Gymnema

Pi 0.0099

MarkerSeek score 69.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1475
variable_sites: 21
pi: 0.0099
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1560-1620 bp	1.0	81.9
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1546-1606 bp	1.0	81.8
ndhC-trnV-UAC_p3	GTCCTTTGTTAATCGGGGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1559-1619 bp	1.0	79.2
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1545-1605 bp	1.0	79.1
ndhC-trnV-UAC_p5	TGGTCCTTTGTTAATCGGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1562-1622 bp	1.0	78.9