Hotspot

psbM-trnD-GUC

LSC · 35016-35857 · 842 bp

Back to Gymnema

Pi 0.00633

MarkerSeek score 64.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 8
pi: 0.00633
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAACG	TTCAATTGGTCAGAGCACCG	55.0	50.0	58.9	58.5	977-985 bp	1.0	70.4
psbM-trnD-GUC_p2	GGCGGTAGGAACTAGAACGA	TTCAATTGGTCAGAGCACCG	55.0	50.0	58.9	58.5	976-984 bp	1.0	70.4
psbM-trnD-GUC_p3	GCGGTAGGAACTAGAACGAA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.1	58.5	975-983 bp	1.0	70.1
psbM-trnD-GUC_p4	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	981-989 bp	1.0	68.8
psbM-trnD-GUC_p5	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.2	58.5	984-992 bp	1.0	68.1