Hotspot

trnH-GUG-psbA

LSC · 77-519 · 443 bp

Back to Gymnema

Pi 0.0411
MarkerSeek score 76.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
443
variable_sites
24
pi
0.0411
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 559-581 bp 1.0 86.1
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 512-534 bp 1.0 86.1
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 573-595 bp 1.0 86.0
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 572-594 bp 1.0 86.0
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 574-596 bp 1.0 86.0