Hotspot

ycf1

SSC · 122989-128361 · 5373 bp

Back to Gymnadenia

Pi 0.00861

MarkerSeek score 44.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5373
variable_sites: 96
pi: 0.00861
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTGCTGTGTCGTACAATGT	GACGAAATAAACCTAGAAGAAATGT	40.0	32.0	55.7	55.7	243 bp	1.0	44.0
ycf1_p2	ATTGCTGTGTCGTACAATGT	ACGAAATAAACCTAGAAGAAATGT	40.0	29.2	55.7	54.6	242 bp	1.0	44.0
ycf1_p3	ATTGCTGTGTCGTACAATGT	AGACGAAATAAACCTAGAAGAAATGT	40.0	30.8	55.7	56.9	244 bp	1.0	43.9
ycf1_p4	ATTGCTGTGTCGTACAATGT	AAGACGAAATAAACCTAGAAGA	40.0	31.8	55.7	52.6	245 bp	1.0	43.9
ycf1_p5	ATTGCTGTGTCGTACAATGT	AGACGAAATAAACCTAGAAGAA	40.0	31.8	55.7	52.6	244 bp	1.0	43.9