Hotspot

rps16

LSC · 4895-6081 · 1187 bp

Back to Gymnadenia

Pi 0.00548

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1187
variable_sites: 13
pi: 0.00548
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGTACTAGTGAGCCTACCC	TCAATTGCATACGGTTCCAA	55.0	40.0	57.2	55.6	1258-1272 bp	1.0	56.9
rps16_p2	AGGTACTAGTGAGCCTACCC	TGCATACGGTTCCAAATTCA	55.0	40.0	57.2	55.6	1253-1267 bp	1.0	56.9
rps16_p3	GGTACTAGTGAGCCTACCCT	TCAATTGCATACGGTTCCAA	55.0	40.0	57.2	55.6	1257-1271 bp	1.0	56.9
rps16_p4	GGTACTAGTGAGCCTACCCT	TGCATACGGTTCCAAATTCA	55.0	40.0	57.2	55.6	1252-1266 bp	1.0	56.9
rps16_p5	GGTACTAGTGAGCCTACCCT	TGTATCGATTCAGCCAATGACT	55.0	40.9	57.2	57.6	1299-1313 bp	1.0	56.4