Hotspot

rpl16

LSC · 80302-81918 · 1617 bp

Back to Gymnadenia

Pi 0.00437

MarkerSeek score 43.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1617
variable_sites: 14
pi: 0.00437
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2172-2198 bp	1.0	82.6
rpl16_p2	TTGGGCACTGCTTCTTTGAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2173-2199 bp	1.0	82.6
rpl16_p3	CGAGCAATCGCACCAAAAAT	AGAGAAGGCAGAGTTCCCTT	45.0	50.0	58.0	58.0	2338-2364 bp	1.0	82.3
rpl16_p4	CGCACCAAAAATCCGAGTTC	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.0	58.0	2330-2356 bp	1.0	82.1
rpl16_p5	TCTCAATTCCCGAGCAATCG	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.1	58.0	2348-2374 bp	1.0	81.9