Hotspot

ndhF

SSC · 109602-111854 · 2253 bp

Back to Gymnadenia

Pi 0.00559

MarkerSeek score 41.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 31
pi: 0.00559
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAAGATTATGATCTGGAGACCC	AATTGGTGAATCGGAAACAA	47.8	35.0	58.2	53.7	2327 bp	1.0	41.1
ndhF_p2	CGAAGATTATGATCTGGAGACCC	TTGGTGAATCGGAAACAATT	47.8	35.0	58.2	53.7	2325 bp	1.0	41.1
ndhF_p3	CGAAGATTATGATCTGGAGACCC	AGAGATCTTAATTGGTGAATCGGA	47.8	37.5	58.2	57.5	2336 bp	1.0	41.1
ndhF_p4	CGAAGATTATGATCTGGAGACCC	TCTTAATTGGTGAATCGGAAACA	47.8	34.8	58.2	56.5	2331 bp	1.0	41.1
ndhF_p5	CGAAGATTATGATCTGGAGACCC	ATTGGTGAATCGGAAACAAT	47.8	35.0	58.2	53.4	2326 bp	1.0	41.1