Hotspot

ndhD

SSC · 114832-116301 · 1470 bp

Back to Gymnadenia

Pi 0.00367

MarkerSeek score 54.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1470
variable_sites: 13
pi: 0.00367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGAAGAATCGTGAAAATATGAAAGT	GCCCGTACTCGAGAAAAGAA	32.0	50.0	56.5	57.6	1669-1670 bp	1.0	40.7
ndhD_p2	CGAAGAATCGTGAAAATATGAAAGTC	GCCCGTACTCGAGAAAAGAA	34.6	50.0	57.5	57.6	1669-1670 bp	1.0	40.7
ndhD_p3	CGAAGAATCGTGAAAATATGAAAGT	TTATTCTTCTCCCGAGCACG	32.0	50.0	56.5	57.4	1643-1644 bp	1.0	40.7
ndhD_p4	CGAAGAATCGTGAAAATATGAAAGTC	TTATTCTTCTCCCGAGCACG	34.6	50.0	57.5	57.4	1643-1644 bp	1.0	40.7
ndhD_p5	CGAAGAATCGTGAAAATATGA	GCCCGTACTCGAGAAAAGAA	33.3	50.0	52.1	57.6	1669-1670 bp	1.0	40.7